{"product_id":"hif1-bata-monoclonal-antibody-e-ab-22189","title":"HIF1 bata Monoclonal Antibody - E-AB-22189","description":"\u003cp\u003eHIF1 bata Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-22189-60, E-AB-22189-120, E-AB-22189-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: HIF-1 β\/ARNT\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: arnT; ARNT protein; ARNT; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor; Dioxin receptor nuclear translocator; Drnt; HIF 1 beta; HIF 1beta; HIF-1-beta; HIF1-beta; HIF1B; HIF1beta; Hypoxia Inducible Factor 1; Hypoxia inducible factor 1 beta; Hypoxia-inducible factor 1-beta; nuclear translocator; Tango\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC-p, IF\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eClone No.: 4C5\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P27540\u003c\/p\u003e\n\n\u003cp\u003eBackground: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin\/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Protein A purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:1000-2000, IHC 1:100-200\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 87kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43148624396467,"sku":"E-AB-22189-60","price":200.2,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43148624429235,"sku":"E-AB-22189-120","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43148624462003,"sku":"E-AB-22189-200","price":558.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_d0f23b08-e075-4e88-923f-02860a749c7a.png?v=1710326920","url":"https:\/\/afsbio.com\/products\/hif1-bata-monoclonal-antibody-e-ab-22189","provider":"AFSBio Inc.","version":"1.0","type":"link"}