{"product_id":"his-tag-polyclonal-antibody-bs67270","title":"His-tag Polyclonal Antibody-BS67270","description":"\u003cp\u003eHis-tag Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS67270-50, BS67270-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/1000 - 1\/2000), IP (1\/10 - 1\/100)\u003c\/p\u003e\n\n\u003cp\u003eBackground: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Anti-His-tag Antibody against HEK293T cells transfected with vector overexpressing His tag (A) and untransfected (B).\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: N\/A\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide HHHHHH.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908740812979,"sku":"BS67270-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908740845747,"sku":"BS67270-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5c7774e1-5917-4132-9083-a7caff182d92.jpg?v=1703006509","url":"https:\/\/afsbio.com\/products\/his-tag-polyclonal-antibody-bs67270","provider":"AFSBio Inc.","version":"1.0","type":"link"}