{"product_id":"his-tag-polyclonal-antibody-bt-ap00862","title":"His-tag Polyclonal Antibody - BT-AP00862","description":"\u003cp\u003eHis-tag Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BT-AP00862-20, BT-AP00862-50, BT-AP00862-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Tags and cell markers; Signal transduction; Epigenetics and nuclear signaling\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: His-Tag\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: His Tag\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"20μL","offer_id":45238870671539,"sku":"BT-AP00862-20","price":151.2,"currency_code":"CAD","in_stock":true},{"title":"50μL","offer_id":45238870704307,"sku":"BT-AP00862-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45238870737075,"sku":"BT-AP00862-100","price":385.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_b9e72936-539a-4663-b972-14f1e8401532.png?v=1769386022","url":"https:\/\/afsbio.com\/products\/his-tag-polyclonal-antibody-bt-ap00862","provider":"AFSBio Inc.","version":"1.0","type":"link"}