{"product_id":"his-tag-rabbit-polyclonal-antibody-es1076","title":"His-tag Rabbit Polyclonal Antibody - ES1076","description":"\u003cp\u003eHis-tag Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1076-50, ES1076-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: 6X His synthetic peptide conjugated to KLH.\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: His-tag,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/1000 - 1\/3000. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 15kD\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: His-Tag\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: His Tag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825535606963,"sku":"ES1076-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825535639731,"sku":"ES1076-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/his-tag-rabbit-polyclonal-antibody-es1076","provider":"AFSBio Inc.","version":"1.0","type":"link"}