{"product_id":"histone-h3-3-rabbit-monoclonal-antibody-bs9952m","title":"Histone H3.3 Rabbit Monoclonal Antibody -BS9952M","description":"\u003cp\u003eHistone H3.3 Rabbit Monoclonal Antibody\u003c\/p\u003e \n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9952M-50, BS9952M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P84243\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF IHC CHIP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 \u003cbr\u003e\nIHC\/ICC\/IF: 1:50-1:200\u003cbr\u003e\nCHIP: 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of Histone H3.3 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Histone H3.3 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: BT-20 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin., ICC staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Histone H3.3; H3F3A; H3.3A; H3F3; ORF Names:PP781; H3F3B; H3.3B\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044035690675,"sku":"BS9952M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044035723443,"sku":"BS9952M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c610c2c7-a0ec-4e9f-804b-f5215fab31e6.jpg?v=1707738824","url":"https:\/\/afsbio.com\/products\/histone-h3-3-rabbit-monoclonal-antibody-bs9952m","provider":"AFSBio Inc.","version":"1.0","type":"link"}