{"product_id":"histone-h3-acetyl-lys23-rabbit-polyclonal-antibody-es1089","title":"Histone H3 (Acetyl Lys23) Rabbit Polyclonal Antibody - ES1089","description":"\u003cp\u003eHistone H3 (Acetyl Lys23) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1089-50, ES1089-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3\/a, Histone H3\/b, Histone H3\/c, Histone H3\/d, Histone H3, H3k23AC\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23. AA range:-9-40\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Histone H3\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Acetyl-Histone H3 (K23) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K23.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Blood, Epithelium, Kidney, Lung, Ovary, Spleen, Uterus,\u003c\/p\u003e \n\n\u003cp\u003eObserved Band (KD): 17kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Neutrophil extracellular trap formation, Alcoholism, Shigellosis, Transcriptional misregulation in cancer, Systemic lupus erythematosus\u003c\/p\u003e\n\n\u003cp\u003eFunction: Caution: Was originally (PubMed: 2587222) thought to originate from mouse\u003cbr\u003e\nDevelopmental Stage: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation\u003cbr\u003e\nFunction: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling\u003cbr\u003e\nmass spectrometry: Monoisotopic with N-acetylserine PubMed: 16457589,Miscellaneous: This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2)\u003cbr\u003e\nPTM: Acetylation is generally l\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Nucleus Chromosome\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: HIST1H3A\/HIST1H3B\/HIST1H3C\/HIST1H3D\/HIST1H3E\/HIST1H3F\/HIST1H3G\/HIST1H3H\/HIST1H3I\/HIST1H3J\/HIST2H3A\/HIST2H3C\/HIST2H3D\/H3F3A\/H3F3B\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Histone H3.1\/Histone H3.2\/Histone H3.3\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 319152\/15077\/15078\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 291159\/100361558\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q6LED0\/P84245\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46314339729587,"sku":"ES1089-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46314339762355,"sku":"ES1089-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies.jpg?v=1793190497","url":"https:\/\/afsbio.com\/products\/histone-h3-acetyl-lys23-rabbit-polyclonal-antibody-es1089","provider":"AFSBio Inc.","version":"1.0","type":"link"}