{"product_id":"histone-h3-di-methyl-k9-polyclonal-antibody-bs94074","title":"Histone H3 (di methyl K9) Polyclonal Antibody-BS94074","description":"\u003cp\u003eHistone H3 (di methyl K9) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS94074-50, BS94074-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P68431(Human) P84243(Human) Q16695(Human) Q6NXT2(Human) Q71DI3(Human) P68433(Mouse) Q6LED0(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000\u003cbr\u003e\nICC:1:100-1:500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Histone H3 (di methyl K9) polyclonal antibody detects endogenous levels of Histone H3 protein only when di-methylated at K9.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: ICC staining Histone H3(di methyl K9) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS., ICC staining Histone H3(di methyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Hist1h3a, HIST1 cluster, H3J, Histone gene cluster 1, H3 histone family, member E, Histone gene cluster 1, H3G, H3 histone family, member J, HIST1 cluster, H3E, HIST1 cluster, H3I, Histone gene cluster 1, H3C, FLJ92264, H3 histone family, member A, H3 histone family, member B, H3 histone family, member C, H3 histone family, member D, H3 histone family, member F, H3 histone family, member H, H3 histone family, member I, H3 histone family, member K, H3 histone family, member L, H3 histone family, member T, H3 histone, family 3A, H3.1, H3.3A, H3\/a, H3\/b, H3\/c, H3\/d, h3\/f, H3\/h, H3\/i, H3\/j, H3\/k, H3\/l, H3\/t, H31, H3F1K, H3F3, H3F3A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1 cluster, H3A, HIST1 cluster, H3B, HIST1 cluster, H3C, HIST1 cluster, H3D, HIST1 cluster, H3F, HIST1 cluster, H3G, HIST1 cluster, H3H, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, HIST3H3, Histone 1, H3a, Histone 1, H3b, Histone 1, H3c, Histone 1, H3d, Histone 1, H3e, Histone 1, H3f, Histone 1, H3g, Histone 1, H3h, Histone 1, H3i, Histone 1, H3j, histone 3, H3, histone cluster 1 H3 family member a, histone cluster 1 H3 family member b, histone cluster 1 H3 family member c, histone cluster 1 H3 family member d, histone cluster 1 H3 family member e, histone cluster 1 H3 family member f, histone cluster 1 H3 family member g, histone cluster 1 H3 family member h, histone cluster 1 H3 family member i, histone cluster 1 H3 family member j, Histone cluster 1, H3a, Histone cluster 1, H3b, Histone cluster 1, H3c, Histone cluster 1, H3d, Histone cluster 1, H3e, Histone cluster 1, H3f, Histone cluster 1, H3g, Histone cluster 1, H3i, Histone cluster 1, H3j, Histone gene cluster 1, H3 histone family, member A, Histone gene cluster 1, H3 histone family, member B, Histone gene cluster 1, H3 histone family, member C, Histone gene cluster 1, H3 histone family, member D, Histone gene cluster 1, H3 histone family, member F, Histone gene cluster 1, H3 histone family, member G, Histone gene cluster 1, H3 histone family, member H, Histone gene cluster 1, H3 histone family, member I, Histone gene cluster 1, H3 histone family, member J, Histone gene cluster 1, H3A, Histone gene cluster 1, H3B, Histone gene cluster 1, H3D, Histone gene cluster 1, H3E, Histone gene cluster 1, H3F, Histone gene cluster 1, H3H, Histone gene cluster 1, H3I, Histone gene cluster 1, H3J, Histone H 3, Histone H3.1, histone H3.1t, Histone H3.2, Histone H3\/a, Histone H3\/b, Histone H3\/c, Histone H3\/d, Histone H3\/f, Histone H3\/h, Histone H3\/i, Histone H3\/j, Histone H3\/k, Histone H3\/l, Histone H3\/m, Histone H3\/o,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Methylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987068194995,"sku":"BS94074-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987068227763,"sku":"BS94074-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a9dea9dd-c648-446f-9e93-016e52fc5c10.jpg?v=1706008392","url":"https:\/\/afsbio.com\/products\/histone-h3-di-methyl-k9-polyclonal-antibody-bs94074","provider":"AFSBio Inc.","version":"1.0","type":"link"}