{"product_id":"histone-h3-di-methyl-lys14-rabbit-polyclonal-antibody-es20556","title":"Histone H3 (Di Methyl Lys14) Rabbit Polyclonal Antibody - ES20556","description":"\u003cp\u003eHistone H3 (Di Methyl Lys14) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES20556-50, ES20556-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: H3K14ME2, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3\/a, Histone H3\/b, Histone H3\/c, Histone H3\/d, Histone H3\/f, Histone H3\/h, Histone H3\/i, Histone H3\/j, Histone H3\/k, Histone H3\/l, HIST2H3A, HIST2H3C, H3F2, H3FM, HIST2H3D, Histone H3.2, Histone H3\/m, Histone H3\/o, H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic Peptide of Histone H3 (Di Methyl Lys14)\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Histone H3\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: The antibody detects endogenous Histone H3 (Di Methyl Lys14) protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500-1000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Blood, Epithelium, Kidney, Lung, Ovary, Spleen, Uterus,\u003c\/p\u003e \n\n\u003cp\u003eObserved Band (KD): 15-17kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Neutrophil extracellular trap formation, Alcoholism, Shigellosis, Transcriptional misregulation in cancer, Systemic lupus erythematosus\u003c\/p\u003e\n\n\u003cp\u003eFunction: Caution: Was originally (PubMed: 2587222) thought to originate from mouse\u003cbr\u003e\nDevelopmental Stage: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation\u003cbr\u003e\nFunction: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling\u003cbr\u003e\nmass spectrometry: Monoisotopic with N-acetylserine PubMed: 16457589,Miscellaneous: This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2)\u003cbr\u003e\nPTM: Acetylation is generally l\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Nucleus Chromosome\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: HIST1H3A\/HIST1H3B\/HIST1H3C\/HIST1H3D\/HIST1H3E\/HIST1H3F\/HIST1H3G\/HIST1H3H\/HIST1H3I\/HIST1H3J\/HIST2H3A\/HIST2H3C\/HIST2H3D\/H3F3A\/H3F3B\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Histone H3.1\/Histone H3.2\/Histone H3.3\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 319152\/15077\/15078\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 291159\/100361558\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q6LED0\/P84245\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46314182508723,"sku":"ES20556-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46314182541491,"sku":"ES20556-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/histone-h3-di-methyl-lys14-rabbit-polyclonal-antibody-es20556","provider":"AFSBio Inc.","version":"1.0","type":"link"}