{"product_id":"histone-h3-rabbit-mab-ac070","title":"Histone H3 Rabbit mAb - AC070","description":"\u003cp\u003eHistone H3 Rabbit mAb\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: AC070-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Loading Control Antibodies\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, IHC-P, ChIP, ELISA\u003c\/p\u003e\n\n\u003cp\u003eCross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)\u003c\/p\u003e \n\n\u003cp\u003eProtein Weight: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved Molecular Weight: 17 kDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide\u003c\/p\u003e\n\n\u003cp\u003eSpecies: Human\u003c\/p\u003e\n\n\u003cp\u003eGeneID: 8290\/8350\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q16695\/P68431\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: H3\/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Histone H3; H3-4; H3\/t; H3\/g; H3FT; HIST3H3\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003ePurity: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Avoid freeze \/ thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eRecommended Dilutions: WB, 1:80000 - 1:320000 IP, 0.5μg-4μg antibody for 100μg-300μg extracts of whole cells IHC-P, 1:5000 - 1:20000 ChIP, 2μg antibody for 10μg-15μg of Chromatin ELISA, Recommended starting concentration is 1 μg\/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (≥1:10000) ，a sequential dilution method is strongly recommended to ensure measurement accuracy.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Cell Cycle, Immunology Inflammation, NF-kB Signaling Pathway.\u003c\/p\u003e\n\n\u003cp\u003eNCBI Alias: H3C1\/H3-4\u003c\/p\u003e\n\n\u003cp\u003eClone Number Info: ARC75100\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"Default Title","offer_id":45190102188211,"sku":"AC070-100","price":252.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_a2444470-6993-4b32-a771-54a470cfe76d.png?v=1766442488","url":"https:\/\/afsbio.com\/products\/histone-h3-rabbit-mab-ac070","provider":"AFSBio Inc.","version":"1.0","type":"link"}