{"product_id":"hla-dob-polyclonal-antibody-bs5758","title":"HLA-DOB Polyclonal Antibody-BS5758","description":"\u003cp\u003eHLA-DOB Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS5758-50, BS5758-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HLA class II histocompatibility antigen, DO beta chain; MHC class II antigen DOB; HLA-DOβ; HLA-DO beta\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P13765\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Peptide (antigen) binding to major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II-Ig dimers and by the activity of MHC molecules HLA-DM and -DO. Accumulating in endosomal\/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: HLA-DOB polyclonal antibody detects endogenous levels of HLA-DOB protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 31 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of HLA-DOB polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: Hela cell lysate\u003cbr\u003e\nLane 2: Raw264.7 cell lysate\u003cbr\u003e\nLane 3: H9C2 cell lysate\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the 43-88 of Human HLA-DOB.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911815336115,"sku":"BS5758-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911815368883,"sku":"BS5758-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_68c9f37a-a9ac-4ffe-913f-ccbe509fd824.jpg?v=1703179872","url":"https:\/\/afsbio.com\/products\/hla-dob-polyclonal-antibody-bs5758","provider":"AFSBio Inc.","version":"1.0","type":"link"}