{"product_id":"hmg-1-rabbit-polyclonal-antibody-es4232","title":"HMG-1 Rabbit Polyclonal Antibody - ES4232","description":"\u003cp\u003eHMG-1 Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES4232-50, ES4232-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],\u003c\/p\u003e \n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: IF, WB, IHC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized peptide derived from the N-terminal region of human HMG-1.\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: HMGB1,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: IF: 1:50-200 Western Blot: 1\/500 - 1\/2000. IHC-p: 1\/100-1\/300. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Ubiquitous. Expressed in platelets (PubMed:11154118).\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): about 30kd\u003c\/p\u003e\n\n\u003cp\u003eFields: Base excision repair, Autophagy - animal, Necroptosis, Neutrophil extracellular trap formation\u003c\/p\u003e\n\n\u003cp\u003eFunction: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Similarity: Belongs to the HMGB family. Similarity: Contains 2 HMG box DNA-binding domains.\u003c\/p\u003e \n\n\u003cp\u003eSubcellular Location: Nucleus Chromosome Cytoplasm Secreted Cell membrane, Peripheral membrane protein, Extracellular side Endosome Endoplasmic reticulum-Golgi intermediate compartment In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: HMGB1\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: High mobility group protein B1\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 3146\u003c\/p\u003e\n\n\u003cp\u003eHuman SwissProt Number: P09429\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 100862258\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: P63158\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 25459\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: P63159\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825503133875,"sku":"ES4232-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825503166643,"sku":"ES4232-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/hmg-1-rabbit-polyclonal-antibody-es4232","provider":"AFSBio Inc.","version":"1.0","type":"link"}