{"product_id":"hmg14phospho-ser21polyclonal-antibody-bs65220","title":"HMG14(Phospho-Ser21)Polyclonal Antibody-BS65220","description":"\u003cp\u003eHMG14(Phospho-Ser21)Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers:BS65220-50, BS65220-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P05114\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC-p, IF\/ICC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/10000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eBackground: high mobility group nucleosome binding domain 1(HMGN1) Homo sapiens The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011],\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003cbr\u003e\nSpecificity: Phospho-HMG-14 (S21) Polyclonal Antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide., Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HMG14 (Phospho-Ser21) Antibody, Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59\u003c\/p\u003e\n\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42895048212659,"sku":"BS65220-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42895048245427,"sku":"BS65220-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_000de3e7-13ac-4429-a398-2f51a41b0c43.jpg?v=1702573039","url":"https:\/\/afsbio.com\/products\/hmg14phospho-ser21polyclonal-antibody-bs65220","provider":"AFSBio Inc.","version":"1.0","type":"link"}