{"product_id":"hmgb1-rabbit-monoclonal-antibody-bs9816m","title":"HMGB1 Rabbit Monoclonal Antibody-BS9816M","description":"\u003cp\u003eHMGB1 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9816M-50, BS9816M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P09429\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:5000 \u003cbr\u003e\nIHC\/ICC\/IF: 1:50-1:200 \u003cbr\u003e\nFC: 1:10-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Research suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, ATF-2, c-Jun and members of the NFκB family to activate transcription.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of HMGB1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of HMGB1 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: MCF-7 whole cell lysate\u003cbr\u003e\n Lane 2: PC12 whole cell lysate\u003cbr\u003e\n Lane 3: F9 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB1 antibody. Counter stained with hematoxylin., ICC staining HMGB1 in NIH\/3T3 cells (green). The nuclear counter stain is DAPI (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004763898035,"sku":"BS9816M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004763930803,"sku":"BS9816M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b5568f47-7c74-44cd-b8a0-97537a3e0d3a.jpg?v=1706445953","url":"https:\/\/afsbio.com\/products\/hmgb1-rabbit-monoclonal-antibody-bs9816m","provider":"AFSBio Inc.","version":"1.0","type":"link"}