{"product_id":"horse-hmgb-2-highmobility-group-protein-b2-elisa-kit-elk9976","title":"Horse HMGB-2 (HighMobility Group Protein B2) ELISA Kit - ELK9976","description":"\u003cp\u003eHorse HMGB-2 (HighMobility Group Protein B2) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9976-48, ELK9976-96, ELK9976-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: High mobility group protein B2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.062 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200847110323,"sku":"ELK9976-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200847143091,"sku":"ELK9976-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200847175859,"sku":"ELK9976-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_79775627-cbdc-4bf6-8d97-f7b6af841e82.png?v=1767065421","url":"https:\/\/afsbio.com\/products\/horse-hmgb-2-highmobility-group-protein-b2-elisa-kit-elk9976","provider":"AFSBio Inc.","version":"1.0","type":"link"}