Horseradish Peroxidase (HRP) is one of the most used enzyme in biochemical applications. It catalyzes the oxidation of a large variety of substrates using Hydrogen Peroxide as oxidizing agent. Biotinylated HRP can used to amplify the signal of certain immunoassays.
Product Data Sheet | Download PDF |
MSDS | Download PDF |
Catalog number: | P2010010 |
Lot number: | 0119P0010 |
Expiration date: | 4/7/23 |
Package Size: | 1 mg |
Specific Activity: | 350 U/mg |
Unit Definition: | Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0. |
Biotin Incorporation: | 1.3 moles biotin/mole HRP (HABA assay) |
Appearance: | Lyophilized powder |
Storage: | -20°C or lower. Avoid repeated freeze-thaw. |
Purity: | >95%. |
Recommended Buffer: | 10 mM citrate, pH 6.0 |