Horseradish Peroxidase (HRP) is one of the most used enzyme in biochemical applications. It catalyzes the oxidation of a large variety of substrates using Hydrogen Peroxide as oxidizing agent. Biotinylated HRP can used to amplify the signal of certain immunoassays.
|Product Data Sheet||Download PDF|
|Package Size:||1 mg|
|Specific Activity:||350 U/mg|
|Unit Definition:||Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0.|
|Biotin Incorporation:||1.3 moles biotin/mole HRP (HABA assay)|
|Storage:||-20°C or lower. Avoid repeated freeze-thaw.|
|Recommended Buffer:||10 mM citrate, pH 6.0|