{"product_id":"hsd3b2-polyclonal-antibody-bs60084","title":"HSD3B2 Polyclonal Antibody-BS60084","description":"\u003cp\u003eHSD3B2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS60084-50, BS60084-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P26439\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: 3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD is the type I protein that catalyzes the oxidative conversion of ∂5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3β-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3β-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: HSD3B2 polyclonal antibody detects endogenous levels of HSD3B2 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of HSD3B2 pAb at 1:500 dilution\u003cbr\u003e\nLane1:A2780 whole cell lysate(30ug)\u003cbr\u003e\nLane2:SK-OVCAR3 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 42 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 3β-HSD; HSD-3β; 3 beta-hydroxysteroid dehydrogenase\/Delta 5--\u0026gt;4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase\/Delta 5--\u0026gt;4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase\u003cbr\u003e\nProgesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase; HSD3B2; HSDB3B\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to residues in Human HSD3B2.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910465523891,"sku":"BS60084-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910465556659,"sku":"BS60084-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2c918092-85bc-49c9-985f-9f3f17436b89.jpg?v=1703092930","url":"https:\/\/afsbio.com\/products\/hsd3b2-polyclonal-antibody-bs60084","provider":"AFSBio Inc.","version":"1.0","type":"link"}