{"product_id":"hsp27-monoclonal-antibody-mb0141","title":"HSP27 Monoclonal Antibody-MB0141","description":"\u003cp\u003eHSP27 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0141-50, MB0141-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04792\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Monkey\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000\u003cbr\u003e\nICC: 1:100~500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of HSP27 and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 23KDa \u003cbr\u003e\nObserved band size: 27KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of HSPB1 in Hela, HsHela, A431, COS7, LNCAP and MCF7 cell lysates and using HSPB1 antibody (1:1000 diluted)., Immunocytochemistry stain of Hela using HSPB1 antibody (1:300).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human HSP27.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989565968563,"sku":"MB0141-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989566034099,"sku":"MB0141-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b5ea1278-f0d1-4f17-80a1-89aef8af92aa.jpg?v=1706095478","url":"https:\/\/afsbio.com\/products\/hsp27-monoclonal-antibody-mb0141","provider":"AFSBio Inc.","version":"1.0","type":"link"}