{"product_id":"human-25-oh-d-25-hydroxy-vitamin-d-elisa-kit-elk0694","title":"Human 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit - ELK0694","description":"\u003cp\u003eHuman 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0694-48, ELK0694-96, ELK0694-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 25 Hydroxy Vitamin D;\u003c\/p\u003e \n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.45 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211521032371,"sku":"ELK0694-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211521065139,"sku":"ELK0694-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200640802995,"sku":"ELK0694-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_fe038f04-b542-444d-b718-9914cad1fdb5.png?v=1767060674","url":"https:\/\/afsbio.com\/products\/human-25-oh-d-25-hydroxy-vitamin-d-elisa-kit-elk0694","provider":"AFSBio Inc.","version":"1.0","type":"link"}