{"product_id":"human-3-mh-3-methylhistidine-elisa-kit-elk11259","title":"Human 3-MH (3-Methylhistidine) ELISA Kit - ELK11259","description":"\u003cp\u003eHuman 3-MH (3-Methylhistidine) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11259-48, ELK11259-96, ELK11259-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 3MH\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 2.61 nmol\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 400 nmol\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 6.25-400 nmol\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolism\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 3-MH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200703062195,"sku":"ELK11259-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200703094963,"sku":"ELK11259-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200703127731,"sku":"ELK11259-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_78e4c38d-39df-4a13-9e78-4f89a7261335.png?v=1767061874","url":"https:\/\/afsbio.com\/products\/human-3-mh-3-methylhistidine-elisa-kit-elk11259","provider":"AFSBio Inc.","version":"1.0","type":"link"}