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Human ABCC3 (ATP Binding Cassette Transporter C3) ELISA Kit

Human ABCC3 (ATP Binding Cassette Transporter C3) ELISA Kit

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Catalogue Number: ELK4733

Alternative Names: ABC31; MLP2; MOATD; MRP3; cMOAT2; Canalicular Multispecific Organic Anion Transporter 2; Multi-specific organic anion transporter D; Multidrug resistance-associated protein 3

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C3(ABCC3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C3(ABCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C3(ABCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C3(ABCC3) in the samples is then determined by comparing the OD of the samples to the standard curve.