{"product_id":"human-acetyl-h3-acetyl-histone-h3-elisa-kit-elk0808","title":"Human Acetyl-H3 (Acetyl Histone H3) ELISA Kit - ELK0808","description":"\u003cp\u003eHuman Acetyl-H3 (Acetyl Histone H3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0808-48, ELK0808-96, ELK0808-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: H3 histone family member E pseudogene; H3 histone family, member A; H3\/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; ; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3\/a; Histone H3\/b; Histone H3\/c; Histone H3\/d; Histone H3\/f; Histone H3\/h; Histone H3\/i; Histone H3\/j; Histone H3\/k; Histone H3\/l;\u003c\/p\u003e \n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.16 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Epigenetics and Nuclear Signaling\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P68431\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212917407923,"sku":"ELK0808-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212917440691,"sku":"ELK0808-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200867688627,"sku":"ELK0808-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_5a570893-98e9-4823-8b25-0db54a31411d.png?v=1767066071","url":"https:\/\/afsbio.com\/products\/human-acetyl-h3-acetyl-histone-h3-elisa-kit-elk0808","provider":"AFSBio Inc.","version":"1.0","type":"link"}