{"product_id":"human-acly-atp-citrate-synthase-elisa-kit-elk0329","title":"Human ACLY (ATP-citRate Synthase) ELISA Kit - ELK0329","description":"\u003cp\u003eHuman ACLY (ATP-citRate Synthase) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0329-48, ELK0329-96, ELK0329-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.52 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabonomics\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACLY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACLY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACLY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACLY in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P53396\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211464966323,"sku":"ELK0329-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211464999091,"sku":"ELK0329-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200860348595,"sku":"ELK0329-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_90ff76af-532d-46bc-9b97-cd9bc36b9819.png?v=1767065731","url":"https:\/\/afsbio.com\/products\/human-acly-atp-citrate-synthase-elisa-kit-elk0329","provider":"AFSBio Inc.","version":"1.0","type":"link"}