{"product_id":"human-anti-asab-anti-sperm-antibody-elisa-kit-elk8071","title":"Human Anti-AsAb (Anti-Sperm Antibody) ELISA Kit - ELK8071","description":"\u003cp\u003eHuman Anti-AsAb (Anti-Sperm Antibody) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8071-48, ELK8071-96, ELK8071-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Anti-Spermatozoa Antibodies; Sperm Antibodies; Antisperm Antibodies\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.19 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Reproductive science;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212336627891,"sku":"ELK8071-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212336660659,"sku":"ELK8071-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200790192307,"sku":"ELK8071-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_55a1a5c0-b853-4b09-ab5c-888d31b182ea.png?v=1767063856","url":"https:\/\/afsbio.com\/products\/human-anti-asab-anti-sperm-antibody-elisa-kit-elk8071","provider":"AFSBio Inc.","version":"1.0","type":"link"}