{"product_id":"human-anti-gad65-anti-glutamate-decarboxylase-65-autoantibodies-elisa-kit-elk0506","title":"Human Anti-GAD65 (Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit - ELK0506","description":"\u003cp\u003eHuman Anti-GAD65 (Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0506-48, ELK0506-96, ELK0506-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: anti-GAD65; anti-Pancreatic Islets And Brain,65kDa; anti-65 kDa glutamic acid decarboxylase; anti-Glutamate decarboxylase 65 kDa isoform\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.26 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 250 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.91-250 IU\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase; Endocrinology; Neuroscience; Autoimmunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GAD65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GAD65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211497930931,"sku":"ELK0506-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211497963699,"sku":"ELK0506-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200636674227,"sku":"ELK0506-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_60e0e25a-3c5c-4bbe-8719-5b570bf46bd9.png?v=1767060531","url":"https:\/\/afsbio.com\/products\/human-anti-gad65-anti-glutamate-decarboxylase-65-autoantibodies-elisa-kit-elk0506","provider":"AFSBio Inc.","version":"1.0","type":"link"}