{"product_id":"human-ap12-apelin-12-elisa-kit-elk1635","title":"Human AP12 (Apelin 12) ELISA Kit - ELK1635","description":"\u003cp\u003eHuman AP12 (Apelin 12) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK1635-48, ELK1635-96, ELK1635-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: APLN12\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 25.28 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 6000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 93.75-6000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Gastroenterology; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211587649715,"sku":"ELK1635-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211587682483,"sku":"ELK1635-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200713646259,"sku":"ELK1635-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_90cadef0-37cd-44a2-89b7-71ff74b7369c.png?v=1767062140","url":"https:\/\/afsbio.com\/products\/human-ap12-apelin-12-elisa-kit-elk1635","provider":"AFSBio Inc.","version":"1.0","type":"link"}