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Human AP2m1 (Adaptor Related Protein Complex 2 Mu 1) ELISA Kit

Human AP2m1 (Adaptor Related Protein Complex 2 Mu 1) ELISA Kit

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Catalogue Number: ELK4489

Alternative Names: AP50; CLAPM1; Clathrin-Associated/Assembly/Adaptor Protein,Medium 1; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Coat Adaptor Protein AP50

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1(AP2m1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1(AP2m1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1(AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1(AP2m1) in the samples is then determined by comparing the OD of the samples to the standard curve.