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Human ASC1 (Asc Type Amino Acid Transporter 1) ELISA Kit

Human ASC1 (Asc Type Amino Acid Transporter 1) ELISA Kit

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Catalogue Number: ELK4616

Alternative Names: SLC7A10; Solute Carrier Family 7 Member 10,Neutral Amino Acid Transporter,y+ System

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asc Type Amino Acid Transporter 1(ASC1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Asc Type Amino Acid Transporter 1(ASC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asc Type Amino Acid Transporter 1(ASC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asc Type Amino Acid Transporter 1(ASC1) in the samples is then determined by comparing the OD of the samples to the standard curve.