{"product_id":"human-baff-cd257-b-cell-activating-factor-elisa-kit-elk2145","title":"Human BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit - ELK2145","description":"\u003cp\u003eHuman BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2145-48, ELK2145-96, ELK2145-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 28 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF\/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF\/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q9Y275\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213202784435,"sku":"ELK2145-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213202817203,"sku":"ELK2145-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200046031027,"sku":"ELK2145-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_e4d60b58-f81f-44f7-945e-c3b6b5edbbf9.png?v=1767031642","url":"https:\/\/afsbio.com\/products\/human-baff-cd257-b-cell-activating-factor-elisa-kit-elk2145","provider":"AFSBio Inc.","version":"1.0","type":"link"}