{"product_id":"human-barbiturates-elisa-kit-elk8382","title":"Human Barbiturates ELISA Kit - ELK8382","description":"\u003cp\u003eHuman Barbiturates ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8382-48, ELK8382-96, ELK8382-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Barbituates\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.1 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 25 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.4-25 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Plasma, Serum, Urine\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212466389171,"sku":"ELK8382-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212466421939,"sku":"ELK8382-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200795697331,"sku":"ELK8382-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_0a971f5d-cc9c-4d2d-ba2d-dde06980e78a.png?v=1767064068","url":"https:\/\/afsbio.com\/products\/human-barbiturates-elisa-kit-elk8382","provider":"AFSBio Inc.","version":"1.0","type":"link"}