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Human BRAF (B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit

Human BRAF (B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit

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Catalogue Number: ELK4445

Alternative Names: B-raf 1; BRAF1; RAFB1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Enzyme & Kinase;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF) in the samples is then determined by comparing the OD of the samples to the standard curve.