{"product_id":"human-c5a-complement-component-5a-elisa-kit-elk1051","title":"Human C5a (Complement Component 5a) ELISA Kit - ELK1051","description":"\u003cp\u003eHuman C5a (Complement Component 5a) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK1051-48, ELK1051-96, ELK1051-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Complement Component 5a\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 28 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Infection immunity; Immune molecule; Autoimmunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P01031\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212957876403,"sku":"ELK1051-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212957909171,"sku":"ELK1051-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45199940812979,"sku":"ELK1051-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_236ec5e0-8a27-4a20-bd19-f88faf57724c.png?v=1767029348","url":"https:\/\/afsbio.com\/products\/human-c5a-complement-component-5a-elisa-kit-elk1051","provider":"AFSBio Inc.","version":"1.0","type":"link"}