{"product_id":"human-cblb-e3-ubiquitin-protein-ligase-cbl-b-elisa-kit-elk0249","title":"Human CBLB (E3 Ubiquitin-Protein Ligase CBL-B) ELISA Kit - ELK0249","description":"\u003cp\u003eHuman CBLB (E3 Ubiquitin-Protein Ligase CBL-B) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0249-48, ELK0249-96, ELK0249-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CBLB; Signal transduction protein CBL-B; SH3-binding protein CBL-B; Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 25 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CBLB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CBLB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CBLB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CBLB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q13191\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212874219699,"sku":"ELK0249-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212874285235,"sku":"ELK0249-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200858677427,"sku":"ELK0249-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_ed7c575e-c0ac-409c-99d2-902ef2dea9d5.png?v=1767065660","url":"https:\/\/afsbio.com\/products\/human-cblb-e3-ubiquitin-protein-ligase-cbl-b-elisa-kit-elk0249","provider":"AFSBio Inc.","version":"1.0","type":"link"}