Catalogue Number: ELK4509
Alternative Names: KATI; GTK; Cysteine-S-conjugate beta-lyase; Glutamine-phenylpyruvate transaminase; Kyneurenine Aminotransferase; Kynurenine Oxoglutarate Transaminase
Assay Type: Sandwich
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic(CCbL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic(CCbL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Conjugate Beta Lyase, Cytoplasmic(CCbL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Conjugate Beta Lyase, Cytoplasmic(CCbL1) in the samples is then determined by comparing the OD of the samples to the standard curve.