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Human CCR5 (Chemokine C-C-Motif Receptor 5) ELISA Kit

Human CCR5 (Chemokine C-C-Motif Receptor 5) ELISA Kit

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Catalogue Number: ELK4497

Alternative Names: CD195; CC-CKR5; CCCKR5; CMKBR5; CHEMR13; HIV-1 fusion coreceptor

Species: Human

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 5(CCR5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 5(CCR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 5(CCR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 5(CCR5) in the samples is then determined by comparing the OD of the samples to the standard curve.