Catalogue Number: ELK4401
Alternative Names: CDC25A; CDC25A2; M-phase inducer phosphatase 1
Species: Human
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research: Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 25(CDC25). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cell Division Cycle Protein 25(CDC25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 25(CDC25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Division Cycle Protein 25(CDC25) in the samples is then determined by comparing the OD of the samples to the standard curve.