For pricing in CAD please contact us

Human ChAT (Choline Acetyltransferase) ELISA Kit

Human ChAT (Choline Acetyltransferase) ELISA Kit

Regular price
$458.00 USD
Regular price
Sale price
$458.00 USD
Unit price
per 
Availability
Sold out

Catalogue Number: ELK4617

Alternative Names: CMS1A; CMS1A2; CHOACTase; Choline acetylase

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Choline Acetyltransferase(ChAT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Choline Acetyltransferase(ChAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Choline Acetyltransferase(ChAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Choline Acetyltransferase(ChAT) in the samples is then determined by comparing the OD of the samples to the standard curve.