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Human CL (Cardiolipin) ELISA Kit

Human CL (Cardiolipin) ELISA Kit

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Catalogue Number: ELK4525

Alternative Names: 1,3-Bis(sn-3-Phosphatidyl)-Sn-Glycerol

Species: Human

Assay Type: Competitive Inhibition

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: tissue homogenates and other biological fluids

Assay Length: 2h

Research: Cardiovascular biology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cardiolipin(CL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cardiolipin(CL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiolipin(CL) in the samples is then determined by comparing the OD of the samples to the standard curve.