Catalogue Number: ELK4526
Alternative Names: TSP1; CRISP-2; GAPDL5; TPX1; CT36; Testis Specific Protein 1; Cancer/Testis Antigen 36; Testis-specific protein TPX-1
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 2(CRISP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 2(CRISP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 2(CRISP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Secretory Protein 2(CRISP2) in the samples is then determined by comparing the OD of the samples to the standard curve.