Catalogue Number: ELK4398
Alternative Names: HCKID; Tau-protein kinase CSNK1D; Casein kinase I isoform delta
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kinase 1 Delta(CSNK1d). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Casein Kinase 1 Delta(CSNK1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kinase 1 Delta(CSNK1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Kinase 1 Delta(CSNK1d) in the samples is then determined by comparing the OD of the samples to the standard curve.