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Human CYP27A1 (Cytochrome P450 27A1) ELISA Kit

Human CYP27A1 (Cytochrome P450 27A1) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4688

Alternative Names: CP27; CTX; CYP27; Sterol 27-Hydroxylase,Cerebrotendinous Xanthomatosis; Vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1(CYP27A1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 27A1(CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1(CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27A1(CYP27A1) in the samples is then determined by comparing the OD of the samples to the standard curve.