Catalogue Number: ELK4441
Alternative Names: EIF4A; BM-010; DDX2B; EIF4F; ATP-dependent RNA helicase eIF4A-2
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 4A2(EIF4A2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 4A2(EIF4A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 4A2(EIF4A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 4A2(EIF4A2) in the samples is then determined by comparing the OD of the samples to the standard curve.