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Human ErbB3 (V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit

Human ErbB3 (V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit

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Catalogue Number: ELK4702

Alternative Names: HER3; MDA-BF-1; C-ErbB3; LCCS2; ErbB3-S; P180-ErbB3; P45-SErbB3; P85-SErbB3; Receptor Tyrosine-Protein Kinase ErbB-3; Lethal Congenital Contracture Syndrome 2

Species: Human

Assay Type: Sandwich

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Tumor immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3(ErbB3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3(ErbB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3(ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3(ErbB3) in the samples is then determined by comparing the OD of the samples to the standard curve.