Catalogue Number: ELK4393
Alternative Names: IRE1; IRE1P; Serine/Threonine-Protein Kinase/Endoribonuclease IRE1; Inositol-requiring protein 1; Ire1-alpha; Serine/threonine-protein kinase; Endoribonuclease
Species: Human
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research: Signal transduction;Apoptosis;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1(ERN1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1(ERN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endoplasmic Reticulum To Nucleus Signalling 1(ERN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endoplasmic Reticulum To Nucleus Signalling 1(ERN1) in the samples is then determined by comparing the OD of the samples to the standard curve.