{"product_id":"human-f-actin-filamentous-actin-microsample-elisa-kit-elk0113ms","title":"Human F-actin (Filamentous Actin) Microsample ELISA Kit - ELK0113MS","description":"\u003cp\u003eHuman F-actin (Filamentous Actin) Microsample ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0113MS-48, ELK0113MS-96, ELK0113MS-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Fibrous Actin\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.18 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.63-40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Developmental science; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200466870451,"sku":"ELK0113MS-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200466903219,"sku":"ELK0113MS-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200466935987,"sku":"ELK0113MS-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_48fe1079-f398-417b-b544-b46114bd7a10.png?v=1767060216","url":"https:\/\/afsbio.com\/products\/human-f-actin-filamentous-actin-microsample-elisa-kit-elk0113ms","provider":"AFSBio Inc.","version":"1.0","type":"link"}