{"product_id":"human-f1-2-prothrombin-fragment-1-2-elisa-kit-elk2028","title":"Human F1+2 (Prothrombin Fragment 1+2) ELISA Kit - ELK2028","description":"\u003cp\u003eHuman F1+2 (Prothrombin Fragment 1+2) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2028-48, ELK2028-96, ELK2028-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: F1+2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.36 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 50 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.79-50 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Hematology\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211603902643,"sku":"ELK2028-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211603935411,"sku":"ELK2028-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200716660915,"sku":"ELK2028-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_d05af5e8-a402-4d4f-aeae-b954764d0016.png?v=1767062239","url":"https:\/\/afsbio.com\/products\/human-f1-2-prothrombin-fragment-1-2-elisa-kit-elk2028","provider":"AFSBio Inc.","version":"1.0","type":"link"}