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Human FcgRT (Fc Fragment Of IgG Receptor Transporter Alpha) ELISA Kit

Human FcgRT (Fc Fragment Of IgG Receptor Transporter Alpha) ELISA Kit

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Catalogue Number: ELK4655

Alternative Names: FCRN; Neonatal Fc receptor; IgG receptor FcRn large subunit p51

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: CD & Adhesion molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Receptor Transporter Alpha(FcgRT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fc Fragment Of IgG Receptor Transporter Alpha(FcgRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fc Fragment Of IgG Receptor Transporter Alpha(FcgRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fc Fragment Of IgG Receptor Transporter Alpha(FcgRT) in the samples is then determined by comparing the OD of the samples to the standard curve.