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Human FKBP4 (FK506 Binding Protein 4) ELISA Kit

Human FKBP4 (FK506 Binding Protein 4) ELISA Kit

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Catalogue Number: ELK4738

Alternative Names: FKBP52; FKBP59; HBI; Hsp56; p52; Rotamase; 51 kDa FK506-binding protein; 59 kDa immunophilin; HSP-binding immunophilin; Peptidyl-prolyl cis-trans isomerase FKBP4

Species: Human

Assay Type: Sandwich

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research: Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 4(FKBP4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FK506 Binding Protein 4(FKBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 4(FKBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 4(FKBP4) in the samples is then determined by comparing the OD of the samples to the standard curve.