Catalogue Number: ELK4456
Alternative Names: FMRP; FRAXA
Species: Human
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research: Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fragile X Mental Retardation 1(FMR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fragile X Mental Retardation 1(FMR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fragile X Mental Retardation 1(FMR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fragile X Mental Retardation 1(FMR1) in the samples is then determined by comparing the OD of the samples to the standard curve.