For pricing in CAD please contact us

Human FMR1 (Fragile X Mental Retardation 1) ELISA Kit

Human FMR1 (Fragile X Mental Retardation 1) ELISA Kit

Regular price
$458.00 USD
Regular price
Sale price
$458.00 USD
Unit price
per 
Availability
Sold out

Catalogue Number: ELK4456

Alternative Names: FMRP; FRAXA

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Reproductive science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fragile X Mental Retardation 1(FMR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fragile X Mental Retardation 1(FMR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fragile X Mental Retardation 1(FMR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fragile X Mental Retardation 1(FMR1) in the samples is then determined by comparing the OD of the samples to the standard curve.