{"product_id":"human-gif-gastric-intrinsic-factor-elisa-kit-elk3212","title":"Human GIF (Gastric Intrinsic Factor) ELISA Kit - ELK3212","description":"\u003cp\u003eHuman GIF (Gastric Intrinsic Factor) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK3212-48, ELK3212-96, ELK3212-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.91 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Endocrinology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P27352\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213313474739,"sku":"ELK3212-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213313507507,"sku":"ELK3212-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200117137587,"sku":"ELK3212-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_e8fa7ece-c16e-48fd-9c89-84faef512cea.png?v=1767033129","url":"https:\/\/afsbio.com\/products\/human-gif-gastric-intrinsic-factor-elisa-kit-elk3212","provider":"AFSBio Inc.","version":"1.0","type":"link"}