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Human GPR78 (G Protein Coupled Receptor 78) ELISA Kit

Human GPR78 (G Protein Coupled Receptor 78) ELISA Kit

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Catalogue Number: ELK4387

Alternative Names: G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: CD & Adhesion molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 78(GPR78). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to G Protein Coupled Receptor 78(GPR78). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 78(GPR78), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 78(GPR78) in the samples is then determined by comparing the OD of the samples to the standard curve.